This book, “Plant Tissue Culture,” has been prepared to provide a comprehensive and systematic account of the principles, techniques, and applications of plant vi tissue culture. The contents are organized in a logical sequence beginning with the fundamental concepts and historical background of the discipline. Early chapters discuss cellular totipotency, laboratory design, sterilization procedures, and the preparation of culture media, all of which form the essential foundation for successful tissue culture work. Subsequent chapters explore key culture systems such as callus culture, cell suspension culture, organogenesis, and somatic embryogenesis, highlighting both their theoretical basis and practical implementation. The book further examines specialized techniques including  micropropagation, meristem culture, anther and pollen culture, haploid production, embryo rescue, endosperm culture, and protoplast isolation and fusion. These techniques have become indispensable tools in plant breeding and genetic improvement programs. Additional chapters address important topics such as somaclonal variation, synthetic seed technology, in vitro conservation, cryopreservation, and the production of valuable secondary metabolites through cultured plant cells.